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KMID : 0358420070500060858
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Korean Journal of Obstetrics and Gynecology
2007 Volume.50 No. 6 p.858 ~ p.864
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Expression of 14-3-3 proteins and Bax in the placenta of preeclampsia
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Park Ji-Kwon
Kim Seon-Im
Paik Won-Young
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Abstract
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Objective: Purpose of our study was to examine the expression level of 14-3-3 proteins and Bcl-2 family and to estimate the interaction between 14-3-3 proteins and Bcl-2 family in normal and preeclamptic placenta.
Methods: Placental tissues were sampled from preeclampsia with caesarean delivery (n=5) and normal pregnant women with caesarean delivery (n=5). Western blot and immunoprecipitation related Western blotting were performed on all placental samples. Unpaired Student t-test was used to determine the statistical significance.
Results: Western blot analysis revealed that the expression of Bax and 14-3-3 zeta was significantly greater in the preeclamptic placenta than in the normal placenta. Immunoprecipitation related Western blotting revealed that the interaction between 14-3-3 zeta and Bax was significantly less in the preeclamptic placenta than in the normal placenta.
Conclusion: Increased expression of Bax and reduced interaction (between) 14-3-3 zeta and Bax in preeclamptic placenta might influence pathogenesis or sequelae of preeclampsia. Further study is needed to identify the trigger that induces dissociation of Bax from 14-3-3 proteins.
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KEYWORD
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preeclampsia, placenta, 14-3-3 proteins, Bax protein
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